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In this research work, the author focuses on the analysis of the regional perspective of the knowledge-based economy and Europe 2020. The Europe 2020 programme is the European Union’s growth strategy for the coming decade and especially for new budget perspective 2014–2020. In a changing world, representatives of the EU want it to become a smart, sustainable, and inclusive economy. These three mutually reinforcing priorities should help the EU and its Member States deliver high levels of employment, productivity, and social cohesion. Concretely, the Union has set five ambitious objectives — on employment, innovation, education, social inclusion, and climate/energy — to be reached by 2020. Each Member State has adopted its own national targets in each of these areas. Concrete actions at EU and national levels underpin this strategy. The main objective of the research task is to give a comprehensive analysis of the knowledge-based economy from the regional perspective and the program Europe 2020. The particular main concern is the increase of the importance of the knowledge-based economy in the region, the Triple Helix model constructed advantages, programme Europe 2020 and financial instrument of this programme — Horizon 2020.